Hi,
I am close to writing up an article which we hope to submit for publication in the coming months. I have 4 rna seq treatments with 3 biological reps each. I've chosen a fairly small list of genes (21) based on Fold change, FPKM , p val and of course biological interest.
What is the accepted protocol for such studies now? Are ppl doing qpcr validation assays? has anyone gone through the review process and been asked to show proof of DE genes. Any input would be greatly appreciated.
thank you.
nsl
I am close to writing up an article which we hope to submit for publication in the coming months. I have 4 rna seq treatments with 3 biological reps each. I've chosen a fairly small list of genes (21) based on Fold change, FPKM , p val and of course biological interest.
What is the accepted protocol for such studies now? Are ppl doing qpcr validation assays? has anyone gone through the review process and been asked to show proof of DE genes. Any input would be greatly appreciated.
thank you.
nsl
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