SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Learning about human population history from ancient and modern genomes. Newsbot! Literature Watch 0 10-18-2011 03:00 AM
PubMed: High-throughput sequencing of complete human mtDNA genomes from the Philippin Newsbot! Literature Watch 0 12-15-2010 11:20 AM
PubMed: State of the art de novo assembly of human genomes from massively parallel se Newsbot! Literature Watch 0 06-01-2010 03:00 AM
De novo assembly of human genomes with massively parallel short read sequencing dan Literature Watch 0 12-21-2009 05:40 AM
PubMed: De novo assembly of human genomes with massively parallel short read sequenci Newsbot! Literature Watch 0 12-19-2009 03:13 AM

Reply
 
Thread Tools
Old 06-04-2010, 11:20 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,438
Default PubMed: Challenges of sequencing human genomes.

Syndicated from PubMed RSS Feeds

Challenges of sequencing human genomes.

Brief Bioinform. 2010 Jun 2;

Authors: Koboldt DC

Massively parallel sequencing technologies continue to alter the study of human genetics. As the cost of sequencing declines, next-generation sequencing (NGS) instruments and datasets will become increasingly accessible to the wider research community. Newcomers are understandably eager to harness the power of these new technologies. Sequencing human genomes on these platforms, however, presents numerous production and bioinformatics challenges. Production issues like sample contamination, library chimaeras and variable run quality have become increasingly problematic in the transition from technology development lab to production floor. Analysis of NGS data, too, remains challenging, particularly given the short-read lengths (35-250 bp) and sheer volume of data. The development of streamlined, highly automated pipelines for data analysis is critical for transition from technology adoption to accelerated research and publication. This review aims to describe the state of current NGS technologies, as well as the strategies that enable NGS users to characterize the full spectrum of DNA sequence variation in humans.

PMID: 20519329 [PubMed - as supplied by publisher]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 08:03 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO