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  • Has anyone done Zebrafish RNA-Seq?

    I have attached RNA seq alignment results for Zebrafish and the % aligned is 65% (and I get 95% alignment with Human samples).

    I wanted to know if you have done Zebrafish RNA-Seq and if you get the same results. These samples were sequenced twice with the same result both times. I don't have anything to compare it too since most of the samples we do are Human samples and Illumina does not zebrafish RNA-Seq data to compare it with.

    These are TruSeq stranded Total RNA libraries using Ribozero H/M/R kit which as per Illumina is compatible with zebrafish.

    Please advice.

    Thanks in advance.
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