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  • Bowtie2 and 2 GB limit

    I don't think I've posted this before.

    I am attempting to do some mapping with bowtie2, and, despite any of my best efforts, I cannot get bowtie2-build to work on anything larger than 2 GB. From the announcement, it appears that the builders say that they've fixed the limitation (presumably by switching to 64 bit variables), but even when I compile with 64 bits, it still spits this error:
    Error: Reference sequence has more than 2^32-1 characters! Please divide the
    reference into batches or chunks of about 3.6 billion characters or less each
    and index each independently.
    has anyone else had any luck?

  • #2
    Out of interest what organism are you working on and how long are your contigs/chromosomes? I'm interested because the BAM index files (BAI) themselves currently impose quite a low limit on reference lengths - something that plant biologists at least may find a problem.

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