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Old 06-03-2013, 02:27 PM   #1
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Location: Merced, CA

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Default Degraded RNA

I am trying to generate transcriptomic libraries from brain tissue. I used a Ribo-zero gold kit to deplete my total RNA. I ran a pico bioanalyzer analysis and found out that most of my samples are degraded. I have very strong bands that are less than 200nt. I would like to use the Scriptseq v2 kit to prepare my libraries for illumina sequencing (100 paired ends). I was wondering if anyone would advise whether to continue this process or consider alternatives at this point. Thanks!

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Old 06-06-2013, 04:56 AM   #2
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Default RNA quality check

Whats the A260/280 ratio of your RNA? Did you have intact ribosomal bands prior to depletion? How about trying a bioanalyzer trace, checking RIN numbers?
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degradation, library prep

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