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Hi, I'm new to RNA-Seq and am looking for some information on the quality/size profile of RNA needed to produce high-quality data on either the 454 or Illumina platform. We are working with some environmental samples, and the goal is to sequence total prokaryotic community RNA. A MoBio soil RNA extraction kit produced decent results from an E.coli test culture but a freshly collected environmental sample looks rather degraded to my eye (see attached Bioanalyzer trace from the RNA Pico kit). I haven't found anything information on how to connect Bioanalyzer trace quality with library outcomes. Can anyone help?
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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