Hi guys, I am new to this site and have a few questions. I have been given a file of sequence reads from Illumina, s_7_sequence.txt in an attempt at de novo assembly (as a means of training me ). I thought my reads were in fastq format, however running velvet said it wasnt. In velvet the only file format that seems to work is gerald format. But when I attempt to run velvetg it says there are no nodes. I think this may be as a result of the file not being in fastq format. My sequences look like this:
HWI-EASXXX_0012:7:1:0:883#CGATGT/1:NACACATACAACACACACAACACACACAACACACAACACACACAA
CACACAACACACACACCACACACACCACAAAACACACAAC: DMZZZVZZZZWZZZZZZZVZZXZXZZZXZZZXZZZXZXZ
XXZZZZZZZRWZZVVXZVZXZZXXNHIUZVVZWXZZWNWXZWRXVV
would anyone know of a script to convert this file format to fastq? Or do you think there is some error as to why velvet is not working on the file?
Thanks in advanced folks !
HWI-EASXXX_0012:7:1:0:883#CGATGT/1:NACACATACAACACACACAACACACACAACACACAACACACACAA
CACACAACACACACACCACACACACCACAAAACACACAAC: DMZZZVZZZZWZZZZZZZVZZXZXZZZXZZZXZZZXZXZ
XXZZZZZZZRWZZVVXZVZXZZXXNHIUZVVZWXZZWNWXZWRXVV
would anyone know of a script to convert this file format to fastq? Or do you think there is some error as to why velvet is not working on the file?
Thanks in advanced folks !
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