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Hi all,
I am new to RNA-seq analysis. So sorry if you feel I am asking a basic question. How to filter raw sequencing reads (74 nucleotides in length) based on their PHRED quality scores to get reads having a certain base-call accuracy, which I can use for alignment to the genomic sequence and then get a list of uniquely mapped, partially mapped and unmapped list.
Thank you
I am new to RNA-seq analysis. So sorry if you feel I am asking a basic question. How to filter raw sequencing reads (74 nucleotides in length) based on their PHRED quality scores to get reads having a certain base-call accuracy, which I can use for alignment to the genomic sequence and then get a list of uniquely mapped, partially mapped and unmapped list.
Thank you