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DNA Barcoding in bacteria
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Try starting with this website: http://www.earthmicrobiome.org/emp-s...protocols/16s/ -
Barcoded pyrosequencing
Thank you sir for your reply, I'll surely follow that link. But I have a doubt, please clarify me.
Can Barcoded pyrosequencing identify bacteria at species level? Because I want to identify bacteria found in human milk at species level by Barcoded pyrosequencing. So, please suggest me sir.
Thank you,
D.E. LebonahComment
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You should be able to identify bacteria at the species level. The barcoding (indexing) will be used to identify the sample source (which individual person, which aliquot) and will not have an influence on the taxonomy. It is the variability in the 16S region that will be the determining factor in attributing species level taxonomy to the obtained sequence data.Originally posted by lebonahd View Post
By the way, the protocol I sent you an URL to is for Illumina (Solexa) sequencing, not Roche's Pyrosequening.
Regards,
CHComment
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