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Mapping SNPs to Chromosomes
I have a reference genome (.fa) and its annotation (.gtf) files. I have sequenced both DNA and RNA from test sample and generated vcf files containing SNP and INDEL locations for each chromosome of the reference genome. I want to plot the identified SNPs and INDELs to identify hot spots on each chromosome. I have read about R being useful to do the same but was unable to narrow down on how to get it done. Please advise.......
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM -
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...03-22-2024, 06:39 AM
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