Hi,
we recently extracted total RNA from mouse tissue (that had a bacterial infection). The bioanalyzer trace for the RNA sample is attached. Can anybody explain what all the peaks are in the trace? Could this be due to the fact that we probably co-extracted eukaryotic (mouse) and prokaryotic (from infection) simultaneously? If that is the case, is there anything that could be said about the quality of this sample.
Illumina recommends to use RNA with an RIN of at least 8. This sample has a RIN of 6.5 but I'm not sure if the RIN in this case accurately reflects the quality of the sample.
Thanks in advance for your help.
Daniela
we recently extracted total RNA from mouse tissue (that had a bacterial infection). The bioanalyzer trace for the RNA sample is attached. Can anybody explain what all the peaks are in the trace? Could this be due to the fact that we probably co-extracted eukaryotic (mouse) and prokaryotic (from infection) simultaneously? If that is the case, is there anything that could be said about the quality of this sample.
Illumina recommends to use RNA with an RIN of at least 8. This sample has a RIN of 6.5 but I'm not sure if the RIN in this case accurately reflects the quality of the sample.
Thanks in advance for your help.
Daniela
Comment