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After PCR enriching some libraries I noticed small peaks of 80 bp in all of my samples (see image). I am using NEB's ChIP-seq kit for Illumina. My inserts are ~65 bp. I see this in my negative control too.
Reading around on the forum it seems that these are likely unreacted/excess primer. I would size select but I'm worried about sample loss since my peak is so small. Do I need to worry about them hybridizing to the flowcell?
If anyone has some insight I'd much appreciate it. Thanks.
Reading around on the forum it seems that these are likely unreacted/excess primer. I would size select but I'm worried about sample loss since my peak is so small. Do I need to worry about them hybridizing to the flowcell?
If anyone has some insight I'd much appreciate it. Thanks.