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  • Qiime parallel_pick_otus_uclust_ref.py

    Good morning everyone!

    I ran parallel_pick_otus_uclust_ref.py with a high quality fasta file and silva.nr.v119.align as the refence file.

    On the QIIME website it says you should get two files - a .txt and a .log file. However, I got a directory file 'POTU_gdTg_'. And when you open this directory you get several files - deletion_list.txt; expected_out_files.txt; merge_map.tt; POTU_gdTg_.0.fasta; POTU_gdTg_.1.fasta; POTU_gdTg_.2.fasta; POTU_gdTg_.3.fasta; POTU_gdTg_.4.fasta; POTU_gdTg_.5.fasta; POTU_gdTg_jobs.txt.

    Does anyone what these files mean? And which would I use to make the OTU tables? Is there another set before I can create the OTU tables? Do I need to assign taxonomy first?

    Thaaaaaanks!!!!

  • #2
    Are you sure this command is still valid?
    Usually, the Qiime OTU tables are in .biom file format since ~2 years.

    Comment


    • #3
      Thank you for your reply!

      Yes - the output of make_otu_table.py is a .biom file. But I don't have the tables - that is what I want to do now!

      I am wondering what file would be the input file for the make_otu_table.py command?

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