Hi all,
We have had sporadic troubles with cluster density. Our samples usually yield between 150.000 and 210.000 colonies per tile (GA IIx).
Last weeks flowcell showed only ~90.000 colonies. Samples originated from different preparations making it unlikely them being the cause.
Our proposed suspects: cluster gen kit / flowcell / sample denaturation.
Has anybody had similar problems or knows which the critical steps in cluster generation are?
We check concentration with bioanalyzer after sample prep; didn't include PhiX.
Thanks
We have had sporadic troubles with cluster density. Our samples usually yield between 150.000 and 210.000 colonies per tile (GA IIx).
Last weeks flowcell showed only ~90.000 colonies. Samples originated from different preparations making it unlikely them being the cause.
Our proposed suspects: cluster gen kit / flowcell / sample denaturation.
Has anybody had similar problems or knows which the critical steps in cluster generation are?
We check concentration with bioanalyzer after sample prep; didn't include PhiX.
Thanks
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