I have a few new questions about enrichment beads that I’m hoping you folks can help me with. In the SV protocols, the number of capture beads in the Lib-A manuals is different from those in the Lib-L manuals (2.0x10^6 vs. 2.4x10^6). Only the SV numbers are different, not MV or LV. Does anyone know if the numbers are actually different, or if this is a typo?
Also, during our training, we were taught to calculate bead enrichment values differently than the manuals describe it, and were given Excel spreadsheets with the calculations we were taught. Basically, the manuals use the enriched beads/total number of input beads; we were taught to use the enriched beads/actual beads recovered. I was wondering how other users are determining enrichment %’s, and what percentage range tends to give good results with the calc we were taught?
Thanks!
Anthony
Also, during our training, we were taught to calculate bead enrichment values differently than the manuals describe it, and were given Excel spreadsheets with the calculations we were taught. Basically, the manuals use the enriched beads/total number of input beads; we were taught to use the enriched beads/actual beads recovered. I was wondering how other users are determining enrichment %’s, and what percentage range tends to give good results with the calc we were taught?
Thanks!
Anthony
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