Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • #16
    Originally posted by cement_head View Post
    So I took another look at this and it strikes me that the whole problem is the use of only four fluors for 16 combinations. (Seems odd that this wasn't the primary issue attempted to be solved; i.e generating 16 distinct fluors.) Once I got that part, it became obvious why there's an issue with colourspace. Curiously, I just found out that MiniSeq and NextSeq from Illumina use only two fluors - seems like a huge potential issue is one isn't resequencing a human genome...
    Yes, if Solid had used 16 colors it might have been substantially better, though that would have added its own unique issues (like potentially taking 4x as long to sequence).

    Illumina's 2-color chemistry is not like Solid Colorspace, though. It's just a binary encoding of bases -> colors; no information is lost (since no two bases share the same pair of color polarities), except that you can no longer distinguish between no signal and one of the bases. It works fairly well in practice (for de-novo sequencing) and you don't need to align sequences to determine what they are. The 2-color platforms have weaknesses, but it is not clear that the weaknesses are linked to the number of dyes.
    Last edited by Brian Bushnell; 07-05-2016, 12:22 PM.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM
    • seqadmin
      The Impact of AI in Genomic Medicine
      by seqadmin



      Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
      02-26-2024, 02:07 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, 03-14-2024, 06:13 AM
    0 responses
    33 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-08-2024, 08:03 AM
    0 responses
    72 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-07-2024, 08:13 AM
    0 responses
    80 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-06-2024, 09:51 AM
    0 responses
    68 views
    0 likes
    Last Post seqadmin  
    Working...
    X