Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • SRA decryption help

    Dear all,

    I have a couple of *.sra.ncbi_enc files and I am trying to decrypt them; however, NCBI website is very confusing:

    1. Here they say that:

    The password is not given on the command line. It must be in a a file that should be able to be read only by the user account running the program.

    The location of that file should be found in one of these places in order:

    1. An environment variable VDB_PWFILE

    2. The VDB Configuration file option 'krypto/pwfile'
    2. Here they say:

    Decryption password and dbGaP repository key.

    Recently implemented “dbGaP repository key” is a dbGaP project wide security token required for running NCBI SRA toolkit and decryption tools. The key is provided in a file with suffix “.NGC”. Currently it needs to be downloaded from “My Research Projects” page of PI’s dbGaP account.

    3. Another Seqnaswers fellow told me to do this which did not work as well:

    1. make a new folder at your home directory: /home/xxx/.ncbi
    2. create a file named vdb-passwd and save your passwd in it
    3. run vdb-decrypt
    Any ideas?

    Thanks,
    GH

  • #2
    Watch this video: https://youtu.be/FjYO6Ys5cpc

    Comment


    • #3
      I get this error while decrypting by VDB-DECRYPT

      err: size "insufficient" while validating file size in file system module

      Comment


      • #4
        vdb-decrypt.2.9.0 err: size insufficient while validating file within file system module - cannot use filesize of zero when checking if cached file is complete

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Essential Discoveries and Tools in Epitranscriptomics
          by seqadmin


          The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...
          Yesterday, 07:01 AM
        • seqadmin
          Current Approaches to Protein Sequencing
          by seqadmin


          Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
          04-04-2024, 04:25 PM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 04-11-2024, 12:08 PM
        0 responses
        45 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 10:19 PM
        0 responses
        46 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-10-2024, 09:21 AM
        0 responses
        39 views
        0 likes
        Last Post seqadmin  
        Started by seqadmin, 04-04-2024, 09:00 AM
        0 responses
        55 views
        0 likes
        Last Post seqadmin  
        Working...
        X