Hello all, I'm very new to this and I may be asking a silly question but I'm generally a bit confused about the adapter molecules and the "key" sequence on the A and B primers. Right, so basically i would like to perform unidirectional sequencing from the A fusion primer on bacterial 16S amplicons using the Lib-A chemistry on the Roche Titanium platform. I have been told that you can remove the 4 bp key from the B adapter molecule on the fusion primer to increase the read length of the sequence of interest. So basically, do I order the A fusion primer (|A adapter sequence|key|forward primer|) and the B fusion primer (|B adapter sequence|reverse primer|) and use the B beads from the kit, then sequence from the A fusion primer towards the bead? The sequencing center will be doing the library prep and sequencing etc, but i just wanted to 1) make sure I understood what was going on, and 2) make sure i order the correct fusion primers!
Cheers everyone,
Dave
Cheers everyone,
Dave
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