Hello.
I'm working with 454 titanium reads for metagenome data.
I've worked with Megan briefly, and it's been great for taxonomic comparison. Since the introduction of version 4, I have had trouble with the functional analysis tools (SEED and KEGG). I've followed the instruction in the manual (blastx, nr or refseq databases) to generate the .rma files. I've tried multiple times, and each time my KEGG and SEED results come back as all hits "not assigned." Is there something I am missing, or are none of my reads really hitting to the databases?
Thanks
I'm working with 454 titanium reads for metagenome data.
I've worked with Megan briefly, and it's been great for taxonomic comparison. Since the introduction of version 4, I have had trouble with the functional analysis tools (SEED and KEGG). I've followed the instruction in the manual (blastx, nr or refseq databases) to generate the .rma files. I've tried multiple times, and each time my KEGG and SEED results come back as all hits "not assigned." Is there something I am missing, or are none of my reads really hitting to the databases?
Thanks
Comment