Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • How to read an Affymetrix Axiom genotype (array data) file?

    Hi,

    I'm trying to put together genotype data for wheat samples from this resource:


    The two types of file I get look like this (array probe set):
    Code:
    Affymetrix Code,Bristol Affy Code,Bristol SNP Code,Bristol Contig Code,Sequence (including SNP ambiguity code)
    AX-94381124,BA00222391,No BS code,GJKKTUG01CGTKM_219,...ACGCA[R]ACNTC...
    AX-94381126,BA00232763,No BS code,FZU8VVO01APRT5_148,...TCACT[Y]GNGTG...
    AX-94381127,BA00233916,No BS code,contig77177_250,...CCCGA[M]NCGAC...
    ...
    and (Array data):
    Code:
    A_mutica,A_speltoides,Adhoc,Ae_caudata,Akteur,A...
    AX-94381124,1,-1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,...
    AX-94381126,1,2,2,1,2,2,2,2,2,2,2,2,2,2,2,2,2,2
    AX-94381127,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2...
    ...
    The only other information provided about the files is this explanation:
    0 = AA, 1 = AB, 2 = BB, -1 = No call

    So the question is, what information is required to map from genotype 'score' to actual genotype? Obviously heterozygous calls are clear and distinguished from homozygous calls, but is A. speltoides AA or GG at AX-94381124?

    Are these actually Affymetrix Axiom format files, or just something custom?


    Thanks for help,
    Dan.
    Homepage: Dan Bolser
    MetaBase the database of biological databases.

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
27 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
30 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
26 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X