what are thoughts on this new method? no need for a covaris, and low input. interested in using before capture protocol, i am concerned the transposome complex will not yield fragments representative of entire genome.
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library prep by transposition
what are thoughts on this new method? no need for a covaris, and low input. interested in using before capture protocol, i am concerned the transposome complex will not yield fragments representative of entire genome.Tags: None
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You should check with your capture reagent vendor -- one of the problems seen with Illumina libraries is "daisy-chaining" of fragments via the adapters which brings in off-target material. Nextera uses custom adapters, so any blocking oligos supplied in the kit won't be correct.
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what about ends fragments ?
Hi all,
This fragmentation seems very interesting by its low input DNA and its relative simplicity!
I am wondering about the non representativity of ends fragments with this fragmentation except with a previous step like circularization or adding primers.
Is that makes sense ?
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