Hi everyone,
my name is Francesca. Yesterday, I completed a new MiSeq run but I had some problems. I use target sequencing technique by Haloplex and I loaded 12 samples. For quantification, I use Tape Station and the results were good. But, the results of MiSeq run was opposite in fact, I obtained a good cluster density 1,337k/mm2 but, the quality was to low 6,1%. I don't understand because I load two of these sempales in other experiments and the results are good.
my name is Francesca. Yesterday, I completed a new MiSeq run but I had some problems. I use target sequencing technique by Haloplex and I loaded 12 samples. For quantification, I use Tape Station and the results were good. But, the results of MiSeq run was opposite in fact, I obtained a good cluster density 1,337k/mm2 but, the quality was to low 6,1%. I don't understand because I load two of these sempales in other experiments and the results are good.
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