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Old 05-26-2010, 01:19 PM   #1
scuellar
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Default Gel-Purify, columns or silica matrix?

Hi,
Iím building some solexa libraries from PCR products and got poor DNA recovering, after gel size selection of linked products, using GFX pharmacia columns. Iím switching to Qiagen columns (QIAquick gel recovery extraction kit) or silica matrix (NucleoTrap gel extraction kit). Any suggestion?

Thanks
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Old 05-26-2010, 01:20 PM   #2
GW_OK
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None of the above. Use Agencourt Ampure beads. They're way, way better than even Qiagen columns.
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Old 05-26-2010, 01:33 PM   #3
scuellar
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Thanks for your fast answer. I'm wondering if which buffer do you use for dissolve the agarose?
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Old 05-27-2010, 06:53 AM   #4
GW_OK
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With the Ampure beads I don't even do gel size selection any more.
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Old 05-27-2010, 10:17 AM   #5
scuellar
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that's nice... thanks
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Old 02-06-2017, 07:44 AM   #6
KimUTA
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I have large and small, unwanted fragments. And an unopened QiaQuick Gel extraction kit. Is the resulting product clean enough for direct use in a library?

I am intrigued by using the AMPure beads to size select. Do you do a 2 step approach, similar to the following NEB protocol? The first bead ratio is used to bind and discard the larger fragments, the second step retains and cleans the desired smaller band. https://www.neb.com/protocols/1/01/0...election-e6270
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Old 02-07-2017, 05:55 AM   #7
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Yep. Works fine.
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