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Old 02-08-2017, 05:23 AM   #1
MisUser
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Question Maximum number of cycles for kit Miseq 50 cycles

Hi sequencing Masters!

What is the maximum number of cycles we can do with a Miseq V2 50 cycles kit. I have been told that there is enough reagents for more than 55 cycles including the 6 cycles for index reading. Can someone confirm this information?
Thank you very much for your support. It is very appreciated.
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Old 02-08-2017, 05:26 AM   #2
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How many are you looking to get?
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Old 02-08-2017, 05:28 AM   #3
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54 maximum + 6 for index
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Old 02-08-2017, 05:32 AM   #4
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It should be fine. Local core does 66 cycle (50x8x8) runs off and on.
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Old 02-08-2017, 05:48 AM   #5
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Great news. Thank you for your answer Genomax!
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Old 02-08-2017, 10:33 AM   #6
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I got an answer from Illumina's tech support:
MiSeq v2 contains enough reagents for 75 cycles of chemistry
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Old 02-14-2017, 08:34 PM   #7
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here you can find the answer:

https://support.illumina.com/bulleti...in-my-kit.html
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