Hi everyone,
Does anyone know if Exo/SAP PCR cleanup is compatible with MiSeq Illuina sequencing?
I am preparing some environmental DNA for MiSeq Illumina sequencing. I have done the first round of PCR with 16S bacterial primers which have overhangs that will be used by the sequencing facility to perform a second round of PCR where they would attach the Illumina adaptors. My question is, is ExoSAP PCR cleanup of this first round of PCR compatible with their 2nd round of PCR and the Illumina MiSeq platform? Is there anything in the PCR reaction (normal PCR reaction performed with 3%v/v DMSO) or in the Exo/SAP reaction, that could interfere with lib preparation or sequencing? Does anyone have experience with this?
Thank you
Does anyone know if Exo/SAP PCR cleanup is compatible with MiSeq Illuina sequencing?
I am preparing some environmental DNA for MiSeq Illumina sequencing. I have done the first round of PCR with 16S bacterial primers which have overhangs that will be used by the sequencing facility to perform a second round of PCR where they would attach the Illumina adaptors. My question is, is ExoSAP PCR cleanup of this first round of PCR compatible with their 2nd round of PCR and the Illumina MiSeq platform? Is there anything in the PCR reaction (normal PCR reaction performed with 3%v/v DMSO) or in the Exo/SAP reaction, that could interfere with lib preparation or sequencing? Does anyone have experience with this?
Thank you
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