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RNA-Seq: Comparative Analysis of RNA-Seq Alignment Algorithms and the RNA-Seq Unified Newsbot! Literature Watch 3 07-31-2011 07:08 PM

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Old 02-14-2019, 07:22 PM   #1
zizi
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Location: Houston, Texas

Join Date: Feb 2019
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Default New to RNA seq.. Need help!!!

Hello All

I am doing RNA seq using KAPA Stranded RNA-Seq Kit with RiboErase. I am following all the recommendations of the protocol but I seem to lose all my RNA during rRNA depletion step


Please suggest what to do.
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Old 02-14-2019, 10:14 PM   #2
luc
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How do you know? I would suggest to follow the library prep protocol all the way to the end and then assess the library or lack thereof.
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