Is it true that single-stranded DNA libraries are initially BOUND directly to the flow cell as suggested in Illumina's diagrams (see panel b in attached figure). I always assumed they simply hybridized to the primer lawns.
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Originally posted by drdna View PostIs it true that single-stranded DNA libraries are initially BOUND directly to the flow cell as suggested in Illumina's diagrams (see panel b in attached figure). I always assumed they simply hybridized to the primer
It seems the description of the initial stages in the this thread aren't quite accurate.
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Originally posted by GenoMax View PostIs this illustration better: http://www.illumina.com/documents/pr...sequencing.pdf
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Originally posted by drdna View PostNo, that's the original image that is also inaccurate for a different reason - see original post. It seems that the Illumina folks did not have anyone who understands the technology check the diagrams coming out of their illustration department/provider. I wanted to use an original Illumina illustration in a talk that describes the principle of bridge amplification but the official diagrams will confuse the audience, so I think I'll create my own.
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