Hi,
We are seeing 65% to 85% duplicate reads consistently in our RNA seq experiments. We use the Truseq and Nugen protocols for the library prep. We have done many RNA seq experiments (both single read, Paired-end) with both Truseq and Nugen protocols and see high duplicate % consistently. I know there have been many discussions about the duplicate reads issue. I would like to know if this is common issue many are facing with RNA seq experiments. I would appreciate your suggestions if any modifications in library prep would improve our results.
Thanks in advance.
We are seeing 65% to 85% duplicate reads consistently in our RNA seq experiments. We use the Truseq and Nugen protocols for the library prep. We have done many RNA seq experiments (both single read, Paired-end) with both Truseq and Nugen protocols and see high duplicate % consistently. I know there have been many discussions about the duplicate reads issue. I would like to know if this is common issue many are facing with RNA seq experiments. I would appreciate your suggestions if any modifications in library prep would improve our results.
Thanks in advance.
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