Hello everyone,
I am currently working with a local BLAST nucleotide database. After getting it set up I am able to BLAST FASTA files without any bother.
What I wanted to do was only search the nt database for viruses.
To this end I have downloaded the virus accession list from NCBI.
I try to use the following command:
$ blastn -db nt -query sequence.fasta -num_alignments 10 -num_descriptions 10 -evalue 1e-6 -gilist viruses.nbr -num_threads 4 -out sequence.tab
When I input this command I get a result saying "Invalid byte in GI list" and the command does not run. Can anyone help me out with this error message? Has there been a problem downloading the accession list file?
Thanks for the help.
I am currently working with a local BLAST nucleotide database. After getting it set up I am able to BLAST FASTA files without any bother.
What I wanted to do was only search the nt database for viruses.
To this end I have downloaded the virus accession list from NCBI.
I try to use the following command:
$ blastn -db nt -query sequence.fasta -num_alignments 10 -num_descriptions 10 -evalue 1e-6 -gilist viruses.nbr -num_threads 4 -out sequence.tab
When I input this command I get a result saying "Invalid byte in GI list" and the command does not run. Can anyone help me out with this error message? Has there been a problem downloading the accession list file?
Thanks for the help.
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