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A recent study published in Veterinary Clinical Pathology by Chu, C. P., & Nabity, M. B., provided a comprehensive assessment of the optimal miRNA isolation and library preparation methods for small RNA‐seq research using canine biofluids. For library prep analysis, they compared the NEXTFLEX® Small RNA-seq kit v3 and the Illumina® TruSeq® Small RNA-seq kit. The authors concluded that the NEXTFLEX® Small RNA-seq kit v3 is the optimal library preparation method for detection of potential biomarkers in canine serum and urine.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...Today, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM