Hi all,
I'm preparing some GBS libraries for Illumina sequencing and have a quick question about gel extraction:
In my old lab we used a blue box transilluminator for cutting bands out of gels for size selection, but I've moved to a lab that only has a UV transilluminator. Will it damage my sequencing library if I expose the gel to UV light while cutting the band? I am concerned about degrading the library or introducing mutations that could get called as SNPs in downstream analyses.
Thank you!
I'm preparing some GBS libraries for Illumina sequencing and have a quick question about gel extraction:
In my old lab we used a blue box transilluminator for cutting bands out of gels for size selection, but I've moved to a lab that only has a UV transilluminator. Will it damage my sequencing library if I expose the gel to UV light while cutting the band? I am concerned about degrading the library or introducing mutations that could get called as SNPs in downstream analyses.
Thank you!
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