Hello,
I test tophat on the test data set, it runs correctly.
But when I run tophat with my single end RNA sequences, tophat_out file only contains logs and tmp, which doesn't have accepted_hits.sam, junctions.bed, insertions.bed and deletions.bed. I don't know why and where is my error.
ps: I just use default parameters.(tophat a_ref a.fq)
Thanks for your help!!!
I test tophat on the test data set, it runs correctly.
But when I run tophat with my single end RNA sequences, tophat_out file only contains logs and tmp, which doesn't have accepted_hits.sam, junctions.bed, insertions.bed and deletions.bed. I don't know why and where is my error.
ps: I just use default parameters.(tophat a_ref a.fq)
Thanks for your help!!!
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