This sounds really cool!
Now if the data is MiSeq and the 250bp reads overlap, should I perform merging first?
Also, what coverage do you suggest the normalization to take place? is 50x enough?
What if my genome is heterozygous. One of the genomes I work with is tetraploid, and some kmers are at a frequency of 25% of those belonging to homozygous regions. How would normalization occur?
Thank you,
Adrian
Now if the data is MiSeq and the 250bp reads overlap, should I perform merging first?
Also, what coverage do you suggest the normalization to take place? is 50x enough?
What if my genome is heterozygous. One of the genomes I work with is tetraploid, and some kmers are at a frequency of 25% of those belonging to homozygous regions. How would normalization occur?
Thank you,
Adrian
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