Hi, good morning,
In my company we are planning to buy a Illumina sequencer to do deNovo sequencing of few animal genomes in a project. It is the first time we do such kind of experiments so we don't have experience in them and we have some doubts to decide which sequencer should we buy.
I understand that we have to buy a sequencer which generates large amounts of long reads with a high coverage, such the HiSeq2500 but perhaps its cost is too high.
I wonder if we could do deNovo assembly with a cheaper sequencer (as the NextSeq500). My question is: since the total output of the sequencer is significantly lower, would it be possible to do deNovo assembly by making some kind of operation to "divide" the genome and sequence a specific part of it in each experiment so that at the end we can assembly all the intermediate results?
Thank you very much
Best regards,
In my company we are planning to buy a Illumina sequencer to do deNovo sequencing of few animal genomes in a project. It is the first time we do such kind of experiments so we don't have experience in them and we have some doubts to decide which sequencer should we buy.
I understand that we have to buy a sequencer which generates large amounts of long reads with a high coverage, such the HiSeq2500 but perhaps its cost is too high.
I wonder if we could do deNovo assembly with a cheaper sequencer (as the NextSeq500). My question is: since the total output of the sequencer is significantly lower, would it be possible to do deNovo assembly by making some kind of operation to "divide" the genome and sequence a specific part of it in each experiment so that at the end we can assembly all the intermediate results?
Thank you very much
Best regards,
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