SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
Stop automatic demultiplexing on MiSeq Heisman Illumina/Solexa 21 03-23-2015 05:42 PM
String of short sequences as a genome for Miseq reporter vl80 Illumina/Solexa 3 03-21-2013 05:12 PM
PC Requirements for MiSeq Reporter and others softwares zgtmann Bioinformatics 5 03-21-2013 06:21 AM
Miseq reporter demultiplexing sunleaf Illumina/Solexa 6 03-11-2013 12:52 PM
Downsample data for MiSeq Reporter kmcarr Illumina/Solexa 7 01-29-2013 04:16 AM

Reply
 
Thread Tools
Old 12-17-2014, 04:37 AM   #1
cinimod
Junior Member
 
Location: Munich

Join Date: Dec 2014
Posts: 3
Default Demultiplexing MiSeq Runs with Miseq Reporter

By default, MiSeq Reporter allows up to 1 mismatch when demultiplexing an indexed MiSeq run. Can I force the software to perform exact matches while demultiplexing the run? Is there an option that can be set in the sample sheet, for example?
cinimod is offline   Reply With Quote
Old 12-17-2014, 06:24 AM   #2
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 6,795
Default

Looking at the MiSeq reporter manual it does not look like there is a way to do that. Have you tried to contact illumina tech support (this may be a non-documented feature)?
GenoMax is offline   Reply With Quote
Old 12-17-2014, 09:58 PM   #3
cinimod
Junior Member
 
Location: Munich

Join Date: Dec 2014
Posts: 3
Default

Thanks for your help, according to the Illumina tech support it is not possible. This is the answer: "MSR demultiplexes with a mismatch of 1. This is not configurable. If you'd like to demultiplex with a different mismatch, I'd advise exploring bcl2fastq1.8.4."
cinimod is offline   Reply With Quote
Reply

Tags
demultiplexing, miseq, mismatches, reporter

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 01:54 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO