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  • Globin reduction for RNA-seq by two oligos

    Dear community,
    we published a detailed globin locking (GlobinLock) methodology to overcome blood sample analysis limitation caused by abundant globin mRNA from erythrocytes. The locking DNA molecules bind specifically at globin RNA poly-A site that is needed for further analysis. Therefore the globin RNAs are “locked” prior downstream manipulations. The GlobinLock is universal for multiple RNA-seq methods like SMART-Seq, G&T-seq, CEL-seq and STRT where anchored oligo-T primer is applied.

    In addition to human, GlobinLock is effective for mouse, rat, cow, dog and zebrafish

    Access to open paper: http://www.nature.com/articles/srep31584
    Attached Files
    Last edited by kaarelkrj; 08-22-2016, 02:38 AM.

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