Some doubts

[suludana]

Hi,

I have some doubts about Illumina-Solexa sequencing that I want to expose:


Which is the function of the long and not complementary part of primer1?

Which are the sequences of the oligos on the flow cell?

What kind of purification I must do in the first PCR product?

Thanks

S

[suludana]

Still...Some doubts

Hi,

I post this thread last week but I did not recive any reply. I insist:


I have some doubts about Illumina-Solexa sequencing that I want to expose:


Which is the function of the long and not complementary part of primer1?

Which are the sequences of the oligos on the flow cell?

What kind of purification I must do in the first PCR product?

Thanks

S

[ECO]

Hey suludana! Thanks for the posts. I've merged them together to avoid clutter, I'm sure someone will answer eventually.

[csquared]

Which is the function of the long and not complementary part of primer1?

Hybridize to the flowcell.

Which are the sequences of the oligos on the flow cell?

See above

What kind of purification I must do in the first PCR product?

Lots of options here. There is the method described in the protocol (Qiagen kit), you can use a magnetic bead protocol or even a precipitation.