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We're currently trying to sequence a number of samples produced with the CleanTag Small RNA Library Prep Kit. We're having some problems, though, since the number of reads we're able to get is abysmally low. Does anyone have any experience with this particular kit? If so, do you have to do anything different, such as use custom sequencing primers? Since this is the only kit producing such issues on our sequencers we suspect there's something particular with it that we're just unaware of.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Today, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM