Hi,
I wonder if anyone knows how one can tell NCBI submission tools to use alternative start codons for an annotated prokaryote genome.
I used GAG to convert the GFF containing the gene predictions into TBL for tbl2asn. I also let it fix the start and stop codons. But a certain amount of genes (10%) apparently use alternative start codons and GAG does not recognize that. tbl2asn then gives a "fatal" error because prokaryote genes are not allowed without start or stop.
Any idea how to fix that?
Thanks!
I wonder if anyone knows how one can tell NCBI submission tools to use alternative start codons for an annotated prokaryote genome.
I used GAG to convert the GFF containing the gene predictions into TBL for tbl2asn. I also let it fix the start and stop codons. But a certain amount of genes (10%) apparently use alternative start codons and GAG does not recognize that. tbl2asn then gives a "fatal" error because prokaryote genes are not allowed without start or stop.
Any idea how to fix that?
Thanks!