Is anyone else using the SPRIworks system with Illumina's TruSeq kits? We are noticing some unfortunate changes. We started using SPRIworks with the old adapters and didn't have much trouble. Now that we are using TruSeq, suddenly the adapter dimer situation is much worse. Also, some libraries are running higher in size than they should be after PCR - for instance, a 200-400 bp size selection is resulting in some libraries with an avg size of 500 bp.
It seems like the size selection is inconsistent.
I'd love to hear any feedback or experiences from other groups.
It seems like the size selection is inconsistent.
I'd love to hear any feedback or experiences from other groups.
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