Has anyone though about how to best design capture probes (we're using Agilent's Custom SureSelect) for sequencing candidate SNPs?
If one designs the probe centered over the SNP then the greatest read coverage with be off either side due to the bimodal distribution of read ends.
Do folks design two capture probes say 100 bp on either side of the target SNP or is this more hassle than it's worth?
If one designs the probe centered over the SNP then the greatest read coverage with be off either side due to the bimodal distribution of read ends.
Do folks design two capture probes say 100 bp on either side of the target SNP or is this more hassle than it's worth?
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