I need to prepare some miRNA libraries for Illumina sequencing, and have been looking at what's available. Does anyone have any experience with any of these kits, good or bad?
-TruSeq Small RNA Sample Prep Kit
-Ambion® RNA-Seq Library Construction Kit
-ScriptMiner™ Small RNA-Seq Library Preparation Kit (EpiCentre)
-NEXTflex™ Small RNA Sequencing Kit (Bioo Scientific)
Also, all the kits seem to require a gel cut following cDNA synthesis. Has anyone used a PippinPrep or SizeSelect gel in lieu of a gel cut? I just hate gel cuts...
Thank you!!!
-TruSeq Small RNA Sample Prep Kit
-Ambion® RNA-Seq Library Construction Kit
-ScriptMiner™ Small RNA-Seq Library Preparation Kit (EpiCentre)
-NEXTflex™ Small RNA Sequencing Kit (Bioo Scientific)
Also, all the kits seem to require a gel cut following cDNA synthesis. Has anyone used a PippinPrep or SizeSelect gel in lieu of a gel cut? I just hate gel cuts...
Thank you!!!
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