Hi,all:
I am doing reads mapping with tophat recently, but i am cufused with the library-type parameter,so i need your helps. My reads was sequenced by the Directional Ligation sequencing (DeLi-seq) strategy, which library-type do i to choose? fr-unstranded or fr-firststrand or fr-secondstrand ? Thank you very much!
best regards!
I am doing reads mapping with tophat recently, but i am cufused with the library-type parameter,so i need your helps. My reads was sequenced by the Directional Ligation sequencing (DeLi-seq) strategy, which library-type do i to choose? fr-unstranded or fr-firststrand or fr-secondstrand ? Thank you very much!
best regards!