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Old 02-15-2013, 12:04 PM   #1
amylee86
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Location: New York

Join Date: Feb 2013
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Default Qubit vs Nanodrop discrepancies??

Hi all,

What kind of difference is expected when quantifying dsDNA on a nanodrop vs. Qubit?

Our core facility has generated Qubit values for my cDNA sampels that are consistently 1.3- to 2.3-fold higher than the Nanodrop values that I generated. I've read a lot of reports claiming that Nanodrop consistently overestimates concentration, so I'm a little puzzled by these Qubit results.

Any help is greatly appreciated!

Thanks in advance,
Amy
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Old 07-10-2013, 08:50 AM   #2
LB2301
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Location: Santiago-Chile

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Default

I have this same problem with my samples!! you managed to solve your doubts? please if your answer is positive, I discuss how? I have this same problem and I think that may be the differences in temperature between rooms!!

thank you
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