Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Contig and scaffold N50

    Hi all,

    Is it possible for contig and scaffold N50s to be very similar (e.g. within 2-3k bp) or the exact same? I have seen a few examples of this in some de-novo assemblies that I am working with and just want to make sure this is OK.

    Thanks for any thoughts!

  • #2
    PacBio assemblies will have contig and scaffold stats that are very close. I guess single-end Illumina or paired-end Illumina on short fragments would also be like that?
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

    Comment


    • #3
      I am using paired end Illumina reads. Usually I see that the contig N50 is 1/2 or 2/3 of scaffold N50, so I was a bit concerned when we got some with exactly the same contig/scaffold N50

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Recent Advances in Sequencing Analysis Tools
        by seqadmin


        The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
        Yesterday, 07:48 AM
      • seqadmin
        Essential Discoveries and Tools in Epitranscriptomics
        by seqadmin




        The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
        04-22-2024, 07:01 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, Today, 06:57 AM
      0 responses
      9 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, Yesterday, 07:17 AM
      0 responses
      13 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 05-02-2024, 08:06 AM
      0 responses
      19 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-30-2024, 12:17 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Working...
      X