Hi experts,
I am new to R and am using DESeq2 to find out differentially expressed genes from aged and young brain sample.
I am using RNA-seq workflow: gene-level exploratory analysis and differential expression.
[ http://www.bioconductor.org/help/wor...seqGene/#count ]
they have given an example wherein they have uploaded .bam files using airway package and then created the count (from .bam file).
I have .bam file generated using illumina basespace, I was wondering how I can use it using DESeq2 pakage in R.
Any suggestions?
Thanks!
I am new to R and am using DESeq2 to find out differentially expressed genes from aged and young brain sample.
I am using RNA-seq workflow: gene-level exploratory analysis and differential expression.
[ http://www.bioconductor.org/help/wor...seqGene/#count ]
they have given an example wherein they have uploaded .bam files using airway package and then created the count (from .bam file).
I have .bam file generated using illumina basespace, I was wondering how I can use it using DESeq2 pakage in R.
Any suggestions?
Thanks!
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