Hi all, I have been reading the information and evaluation of the potential utility of MinION these days. I would like to see if there is any update in the throughput of MinION recently.
From the ONT website, the R9 flowcell lifespan is 48 hours. So, anyone could share your experience on the total yield of the flowcell from your real data? Is there any improvement on the yield for the latest flowcell which can be purchased from the website? Would the flow cell performance deteriorate over time? I read from some posts the performance deteriorate after 6 hours.
So if I would like to increase the yield, can I adjust the speed the DNA passes through the nanopore? but I don't know if it would then be any tradeoff between the read quality and the fast mode.
I really appreciate any help you can provide.
From the ONT website, the R9 flowcell lifespan is 48 hours. So, anyone could share your experience on the total yield of the flowcell from your real data? Is there any improvement on the yield for the latest flowcell which can be purchased from the website? Would the flow cell performance deteriorate over time? I read from some posts the performance deteriorate after 6 hours.
So if I would like to increase the yield, can I adjust the speed the DNA passes through the nanopore? but I don't know if it would then be any tradeoff between the read quality and the fast mode.
I really appreciate any help you can provide.
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