splitting files

[skoyama]

I`m working on human heart RNAseq datasets GSE71613
These files are described as paired end data.

So I downloaded files and decompressed by SRE toolkit with --split-3
One of extracted data (example_1) shows relatively good quality, but the others shows low quality and its cycle-quality distribution seems to be strange (example_2)...

What happens?

Example_1


Example_2

[dpryan]

Looks like there was a bubble or some major issue in read 2. Truncate read 2 to 70 bases before using the dataset.