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Old 02-11-2014, 09:25 AM   #1
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Location: Stanford

Join Date: Jun 2009
Posts: 181
Default ERCC ExFold vs. regular spike-in mix?

Does anyone know the practical difference between the Ambion ERCC RNA Spike-In Mix (Life Tech #4456740) and their ExFold mixes (#4456739)? The regular mix gets you "absolute quantification", which is nice. I know the ExFold mixes also let you standardize your differential-expression results somehow, but what can you actually do with this information?
jwfoley is offline   Reply With Quote
Old 04-21-2014, 11:22 AM   #2
Location: SF Bay Area

Join Date: Feb 2012
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The practical difference between the mixes is that most of the spike ins are at different concentrations between mixes.

You can use this difference to determine things - particularly detection limits for varying ratios. This provides you with some valuable ways to prune your gene lists - your spike-ins become "true positives" for DE, in addition to "true negatives".
jparsons is offline   Reply With Quote

control, ercc, spike-in

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