My latest RNA Bioanalyzer runs produced unexpected results, with migration time being reduced for both the ladder and the samples, see attached profiles for comparison (below in this order: abnormal ladder and an example of a sample in that run, normal ladder and the same sample).
I tried different gel preparations, and got similar results, which I believe affects proper quantification (but maybe still okay to check RNA integrity?). I've seen explanations for slow migrating fragments, but I haven't seen reports of what I have. Note that it's insect RNA, which is why there is only one single peak apart from the marker.
Does anyone ever had that problem? Cheers!
I tried different gel preparations, and got similar results, which I believe affects proper quantification (but maybe still okay to check RNA integrity?). I've seen explanations for slow migrating fragments, but I haven't seen reports of what I have. Note that it's insect RNA, which is why there is only one single peak apart from the marker.
Does anyone ever had that problem? Cheers!
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